Double Beam UV Visible Spectrophotometer BHV1E1 (BSDBU-301)

Spectrophotometer, Double Beam Spectrophotometer

• Sea, Air, Door to Door Shipping
• 1 Year Warranty
• US & European Standards

Specifications

Model	BHV1E1
Wavelength Range	190-1100 nm
Spectral Bandwidth	1.8 nm
Optical System	Double Beam, Grating 1200 lines/mm
Wavelength Accuracy	±0.3 nm
Wavelength Repeatability	0.2 nm
Scan Speed	Hi, MED., LOW., MAX. 3000 nm/min
Photometric Accuracy	±0.3 %T or ±0.003A @1A
Photometric Range	0-200%T,-0.3-3A
Stray Light	0.04%T @220 nm, 360 nm
Stability	0.0003 A/h @500 nm
Display	5 inches LCD (320x240 dots)
Baseline Flatness	±0.0005 A
Standard Cell Holder	Standard 10 mm single cell holder (2 pcs)
Sample Compartment	Standard 10 mm pathlength cuvette
Light Source	Tungsten & Deuterium lamp (Pre-aligned)
USB Type A Port	USB Type A for USB memory device (Right side)
USB Type B Port	USB Type B for optional computer connectivity (Back)
Output Parallel Port	Parallel port for printer
Power Requirement	AC 110/220 V 50/60 Hz
Dimensions (LxWxH)	589x428x200 mm
Weight	22 kg

Description

It is an advanced double beam design consisting of 3 models.
The two detectors measure sample and reference respectively and simultaneously for optimizing measurement accuracy.
They provide excellent performance for measurements in the range of 190 to 1100 nm, the memory is 32K. They are suitable for pharmaceutical, biochemical and clinical lab applications as well as routine applications such as quantitative analysis, kinetics, wavelength scan, multiple components and DNA/Protein. PC Windows application software makes these instruments versatile. All instruments provide excellent performance for measurements.

Features

Fixed or variable slits (Bandwidths).
For stand-alone models, all software methods are included as built-in standard; this eliminates the need of software.
Online software upgrading via the internet helps to keep it updated.
Data Download-to-PC software expands the data storage to unlimited.
The stand-alone model has a 5-inch screen and the PC model has UV-Vis Analyst software.
Data can be saved by a USB memory device directly.
Stand-alone models of UV-6 Series have the same functions as UV-3 series; see next page for details.


Brief Introduction:
All methods are included as built-in standard; this eliminates the need for software. Online software update via the internet.
The local control software includes functions such as: Photometry, Quantitative, Wavelength Scan, Kinetics, DNA/Protein, Multi-wavelength Test and System Utilities.


Standard Curve:
Up to 10 standard solutions may be used to establish a calibration equation curve. There is a choice of four methods for fitting curves through the calibration points: Linear fit, Linear fit through zero, square fit and cubic fit.


Multi-Wavelength:
Up to 10 wavelengths may be entered, allowing the measurement of multiple wavelengths on a series of samples.


Wavelength Scan:
The Wavelength Scan intervals are 0.1, 0.2, 0.5, 1, 2, 5 nm, and High, Medium and Low scan speeds are available. Scan speeds vary from 100 to 3000 nm/min. Wavelengths are scanned from high to low so that the instrument stands-by at high wavelength. This minimizes the degradation of UV sensitive samples. Precise control of filter and lamp changes means that their effects are not seen on the final scan. Post-run manipulation includes re-scaling axes, curve tracking and peak picking.


Kinetics:
This mode may be used for scanning time courses or reacting rate calculations. Abs. VS Time graphs are displayed on the screen in real time.
Wait time and measurement time up to 12 hours may be entered with time intervals of 0.5, 1, 2, 5, 10, 30 seconds and 1 min.
Post-run manipulation includes re-scaling, curve tracking and selection of the part of the curve required for the rate calculation. Rate is calculated using a linear regression algorithm before multiplying by the entered factor.


DNA/Protein Test:
Concentration and DNA purity are calculated by Absorbance ratios 260 nm/280 nm or 260 nm/230 nm with optional subtracted absorbance at 320 nm.
DNA Concentration = 62.9A260 - 36.0A280 or 49.1A260 - 3.48A230
Protein Concentration = 1552A260 - 757.3A280 or 183A220 - 75.8A230
Other wavelengths and factors may be entered.



UV Analyst PC-Control Software:

The PC application software offers:
Photometric Mode
Quantitative test (Standard curve)
Wavelength Scan
Kinetics
DNA/Protein
Multi-Wavelength
System Utility
The PC application software UV-Vis Analyst takes the best features of the stand-alone version plus more powerful data processing, expanded data collecting, and storage capability. It comes standard with UV3/6 series PC models and is optional to stand-alone models.

Quantitative Test (Standard curve):
Use up to 20 standards to establish a standard curve. Four methods for fitting a curve:
Linear fit.
Linear through zero.
Square fit.
Cubic fit.


Multi-wavelength:
Up to 20 wavelengths can be selected and multiple samples can be measured. (Auto cell changer is required to run multiple samples automatically)


Wavelength Scan:
Automatically record peaks and valleys. The quantity of channels is unlimited; you can simultaneously store as many as desired.
Post-run manipulation and processing includes:
Re-scaling axes, curve.
1 to 4 derivative.
Smoothing, combination, zooming, overlap.


Kinetics (Abs. VS Time):
The Kinetics mode may be used for scanning time courses or reacting rate calculations. Abs. VS Time graphs are displayed on the screen in real time. Wait time, measurement time and time intervals may be entered.
Post-run manipulation includes re-scaling, curve tracking and selection of the part of the curve required for the rate calculation. Rate is calculated using a linear regression algorithm before multiplying by the entered factor.


DNA/Protein:
Concentration and DNA purity are quickly and easily calculated:
Absorbance ratios 260 nm/280 nm with optional subtracted absorbance at 320 nm.
DNA Concentration = 62.9A260 - 36.0A280
Protein Concentration = 1552A260 - 757.3A280
Other wavelengths and factors may be entered.

Accessories For Purchase

images	name	og_model
	Micro Cell Holder (Beam height: 15mm)	900240
	8-Position Auto Cell Changer	900310
	4-Cell Holder for 10mm SQU.cuvette	900410
	4-Cell Holder for 50mm SQU.cuvette	900420
	4-Cell Holder for 100mm SQU.cuvette	900430
	Square Cuvettes Glass :10 mm	916101 QUARTZ: 10 mm 916111 20 mm 916112 30 mm 916113 50 mm 916114 100 mm 916115
	20 mm	916102
	30 mm	916103
	50 mm	916104
	100 mm	916105
	Square cuvettes Quartz:10 mm	916111
	20 mm	916112
	30 mm	916113
	50 mm	916114
	100 mm	916115
	Micro cell, Quartz (Beam height: 15mm) 100UL	916126
	200UL	916127
	500UL	916123
	Sipper System	900140
	Constant-Temperature System	900150
	Constant-Temperature Sipper System	900160
	Test Tube Holder	900530
	Cylindrical Cell Holder	900540
	Solid Sample Holder (Single Cell)	900550
	Water-Jacketed Cell Holder	900610
	10mm Water-Jacketed 4-Cell Holder	900620
	Milas Deuterium Lamp	916633
	Halogen Lamp(Philips)	6V10W: 911634
	Halogen Lamp(Philips)	12V20W: 916634
	Halogen Lamp(Osram)	961634
	Self Masking Cont. Flowthrough G.Cell (Beam height: 15mm) 5mm	916135
	10mm	916136
	20mm	916137
	30mm	916138
	Self Masking Cont. Flowthrough Q. Cell (Beam height: 15mm) 5mm	916145
	10mm	916146
	20mm	916147
	30mm	916148
	Thermal Printer	920910

Operating Manual

Download


1.Safety
2.Package Contents
3.Unpacking
4.Installation
    4.1. Environment Required
    4.2. Install Spectrophotometer
5.Overview
6.Symbols
7.Main Specifications
8.Description of Appearance and Keys
    8.1.Appearance
    8.2.Keypad
    8.3.Description of Keys
9.Functions
    9.1.Photometry
    9.2.Quantitation
    9.3.Wavelength Scan
    9.4.Kinetics
    9.5.DNA/Protein Measurement
    9.6.Multi-wavelength
10.Getting Started
11.Important Guidelines
12.General Operating
13.Measuring
    13.1.Photometry
    13.2.Quantitation
    13.3.Wavelength Scan
    13.4.Kinetics
    13.5.DNA/Protein Mode

1.Safety

Please follow the guidelines below, and read this manual in its entirety to ensure safe operation of the unit.

We recommends against the use of BSDBU-301 Spectrophotometer.

Do not open the device.

Disconnect the device from the mains supply before carrying out maintenance work or changing the fuses.

The inside of the device is a high-voltage area Danger!

Do not use the device if it is damaged, especially if the main power cable is in any way damaged or defective.

Repairs may only be carried out by the service technicians from us and authorized contractual partners.

The device must be connected to a power outlet that has a protective ground connection.

If the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired.

Do not allow any liquid to enter into the device.

Do not operate the device in a hazardous location or potentially explosive environment.

2.Package Contents

Description	Quantity
Spectrophotometer	1PC
10mm Glass Cuvette	4PCS
10mm Quartz Cuvette	2PCS
Power Cord	1PC
User's Manual	1PC
Dust Cover	1PC

3.Unpacking

Open the package, according to carefully check the packaging packing list items, if found inside the packaging are missing or damaged items please

contact us and authorized contractual partners.

4.Installation

4.1. Environment Required

To ensure the best performance, the following conditions are required:

The best working temperature range is 16-35°C and the humidity is 45-80%.

Keep it as far as possible away from the strong magnetic or electrical fields or any electrical device that may generate high-frequency fields.

Set the unit up in an area that is free of dust, corrosive gases and strong vibrations.

Remove any obstructions or materials that could hinder the flow of air under and around the instrument.

The power requirement is 110±11V/60±1Hz or 220±22V/50±1Hz.

Use the appropriate power cord and plug into a grounded outlet.

If the local voltage is not stable, a voltage regulator is required.

Be away from direct sunlight.

4.2. Install Spectrophotometer

Placement

Place the instrument on the stable table carefully.

Install Printer (Printer is Optional Accessories)

Check to confirm instrument power switch is turned off, connect the printer's data cable to the instrument's parallel port.

Link the Power Cord

Check to confirm instrument power switch is turned off, the power cord plug into two separate power interface and power supply socket apparatus.

5.Overview

BSDBU-301 Spectrophotometer is an electrical measure instrument which is widely used in the laboratories.

Use Frequency:Intermittence

Excessive Voltage(Current):No

Pollution Class:Class 1

6.Symbols

The following chart is an illustrated glossary of the symbols that are used in this manual.

Caution, Danger!

Caution, High Voltage!

Caution, Hot!

Ground

Fuse

Recycle, this instrument will be called back by the appointed Electrical Treatment Department or by the original Manufacturer when wasted.

7.Main Specifications

Optical SystemDouble beam

Wavelength Range190-1100nm

Wavelength Accuracy±0.3nm

Wavelength Repeatability0.2nm

Photometric Range-0.3-3A, 0-200%T

Photometric Accuracy±0.3%T

Photometric Repeatability0.2%T

Spectral Bandwidth1.8nm

Stray Light0.05%T@220nm&360nm

Stability±0.001A/h@500nm

Work Mode

Photometry, Quantitation, Kinetics, Wavelength Scan, DNA/Protein, Multi Wavelength

Interface USB, Parallel(printer)

Power Requirement AC 110/220V, 50/60Hz

Dimensions 600x450x260

Weight 20kg

Work Environment 15-35°C, 15-70% relative humidity

Store Environment -10-50°C, 15-70% relative humidity

8.Description of Appearance and Keys

8.1.Appearance

Front View

Back view

1 LCD Display Lid of Sample Room

2 Sample holder

3 LCD Display

4 Keypad

5 USB Port(Type A, USB Memory)

6 Cover of Fan

7 Power Switch

8 Power Socket

9 LCD Contrast Adjust

10 Printer port

11 USB port (Type B, Communication)

8.2.Keypad

8.3.Description of Keys

Function Keys: Functions on-screen prompts

… Numeric Keys: Enter numbers and letters

CLEAR Key: Delete the input value or stored data

ESC Key: Return to previous Interface

100%T/0Abs Key: Blank

OPEN Key: Open files stored in internal memory

SAVE Key: Save files to internal memory

START/STOP Key: Start/Stop testing

GOTO ʎ Key: Set wavelength

PRINT Key: Print measuring result

ENTER Key: Confirm operation

CELL Key: Select/Deselect Auto-cell Holder

RIGHT, LEFT Keys: Search peak/valley and set X scale

UP, DOWN Keys: Scroll menu/data and set Y scale

9.Functions

9.1.Photometry

Display results as Abs, %T or Concentration.

9.2.Quantitation

We provide 2 methods to establish a Standard Curve:

2 methods to set up the regression curve.

Up to 10 standard samples to calibrate the regression curve.

3 methods for curve fit.

Linear fit, Quadratic fit and Cubic fit.

9.3.Wavelength Scan

Allow user to set scan step (0.1, 0.2, 0.5, 1.0 and 5.0nm).

Spectrum display mode can be changed (Wavelength-%Transmittance or Wavelength-Absorbance).

9.4.Kinetics

Allow user to set scan Interval (0.5, 1.0, 2.0, 5.0, 10, 30 and 60s).

Spectrum display mode can be changed (Time-%Transmittance or Time-Absorbance).

9.5.DNA/Protein Measurement

Wavelength points and ratios can be set up.

Results will be grouped into a table format automatically.

9.6.Multi-wavelength

Up to 10 wavelength points can be set up.

Results will be grouped into a table format automatically.

10.Getting Started

The following chart describes the basic operation of the instrument.

Turn On and Self-check

Switch on the power. Then the instrument begins to self-check and 15 minutes' warm up.

Self-check includes the following steps:

Turn on lamps → Check Sensor → Initialize AD → System position → Get Dark Current → Warm up.

Finished self-check, "System calibration" interface displays, press the keys , to select to do calibration or not.

After system do or skip calibration, instrument displays Main Interface.

11.Important Guidelines

Reagents and dilution buffers can cause cauterization and other damage to health.

Samples (nucleic acids, proteins, bacteria cultures) can be infectious and cause serious damage to health.

During sample preparation, measuring procedures and maintenance and cleaning work, observe all local laboratory safety precautions (e.g. wear protective clothing and gloves, use of disinfectant) regarding the handling of sample material.

Dispose of measuring solutions and cleaning and disinfectant materials in accordance with the relevant local laboratory regulations.

12.General Operating

Select Application

Main menu, press numeric keys or use the key , to choose corresponding menu, then press to enter into.

Set Wavelength

Press to set wavelength, use numeric keys to input the values, press to confirm and go to the point you set, then do blank automatically.

Set Parameters

Press function key to set parameters, press , to choose or input the values by numeric keys, press to enter into, press to return.

Set Auto-cell Holder(Optional) Press to active the auto cell holder and press the numeric key (1-8)to make corresponding cell position at the light path. Press again to inactive the auto cell holder.

Delete the Input Value

Press to delete a character, press to delete all the characters.

Delete the Test Results and Stored data

Press to delete the test result or stored data.

Blank

Put the Reference in the light path, press to do blank.

Measure Samples

Put the samples in the light path, press to measure.

Print the Test Results

Store the Test Results

Press to store the test results, input the file name by numeric keys

and press to save. If the USB Disk was installed, the data will save

in the USB Disk, or the data will save in the internal memory.

Load the Stored File

In the test interface, press to go into file selecting interface,

press , to choose the file you want, press to open. If the USB Disk was installed, the data will open from the USB Disk, or the data will from the internal memory.

13.Measuring

13.1.Photometry

Step 1.Start Photometry

Main menu, press numeric key Or , to choose "Basic Mode", then press

.

Step 2.Set Photometric Mode

Press to set photometric mode. Press , to choose "Abs.", "T%" or "Conc./Factor" and press to confirm. If users choose "Abs." or "T%", please go to step 5 directly.

Step 3.Set Concentration Unit

Press to set concentration unit. Press , to choose unit followed with pressed to confirm. You can also choose "other" to input the unit.

Step 4. Set "Factor" or "Standard"

Two methods are under your choice:

Method 1:Input Factor F

Press to set F. Input the value of F by numeric keypad, press to confirm. Then the F value would display on the screen.

Method 2:Use Standard Sample

Put the reference sample in the light path and calibrate 100%T/0Abs; Put the standard sample in the light path,press to start the mark. Input the concentration value of the standard and press to confirm, then it displays on the screen.

Step 5.Set Wavelength

Press to set wavelength, input the value by the numeric keypad

followed with Pressed to confirm.

Step 6. Measure samples

Put the sample to be measured in the main light path and put Reference in the reference light path, then the result displays on the screen automatically.

13.2.Quantitation

Step 1. Start Quantitation

Main menu, press or press , to choose "Quantitative Mode"

followed with Pressed to confirm.

Step 2. Set Unit

Press to set concentration unit, press , to choose and press to confirm.

Step 3.Establish Standard Curve or load the stored curve

Press to go into set up interface, 2 methods are under your choice.

Establish Standard Curve:

Method 1: Input Regression Equation

1) Set Fit Curve Method. Press to set Fit method,use ,

to choose the method and press to confirm.

2) Set Wavelength. Press to set wavelength. Use , to choose measure method, then press to confirm. Input the wavelength value you need and press to confirm.

3) Setup Standard Samples. Press to setup standard, input the

concentrations of corresponding standard samples according the

indication and press to confirm. Users can use , to

choose the value you just input and press to delete, then

input a new value, press to confirm. Press to cancel

after all the input.

4) Calibrate Standard Samples. Put the corresponding standard samples in the main light path and put Reference in the reference light path as the screen indicates and press to measure. Then the Abs. value would appear in the corresponding table.Load the Stored CurvesIn the "Calibration Table"interface, press to go into files select interface. Use , to select the curve you need and press to load.

Load the Stored Curves

In the "Calibration Table" interface, press to go into files select interface. Use , to select the curve you need and press to load. Users can press to view the curve.

Step 4. Return the sample measurement interfaceIn the "Calibration Table" interface, press to return the Quantitative Test interface.

Step 5. Measure Samples

Place the sample to be tested in the light path, press to measure.Then the test result will display in the data sheet. Repeat this step to finish measuring all the samples.

13.3.Wavelength Scan

Step 1. Enter into Wavelength Scan

Main menu, press numeric key or , to choose "Wavelength Scan" and press to enter.

Step 2. Parameters Setup

Press to set parameters, set "Scan From" , "scan to", "scan step"and "scan speed", press to confirm.

Step 3. Set Photometric Mode

Press to set photometric mode,choose "T%", "Abs." or "E" and press to confirm.

Step 4. Scan Samples

Put the sample to be measured in the main light path and put Reference in the reference light path, press to scan the sample, press to cancel.

Step 5. Search Peaks

After scanned, press to go into peak search mode. Press to set peak height, input the peak height and press to confirm.Press , to display the value of every wavelength point. Press , to display the value of every peak.

Step 6. Smooth the Curve

After scanned, if there are many burrs on it, press to smooth the curve.

13.4.Kinetics

Step 1. Enter into Kinetics

Main menu, press or , to select "Kinetics Mode" and press to confirm.

Step 2. Setup Parameters

Press to set parameters, input the corresponding values of"Total Time", "Delay Time" and "Time Intervals" according the screen indicates. Press to confirm.

Step 3. Set Photometric Mode

Press to set photometric mode, choose "T%" or "Abs." and press to confirm.

Step 4. Set Wavelength

Press to to set wavelength, input the value of the wavelength by numeric keypad and press to confirm

Step 5. Measure Samples

Put the sample to be measured in the main light path and put Reference in the reference light path, press to begin the test, press to cancel.

Step 6. Calculate Response Rate

After time scanned, if users want to calculate the response rate of some period, you can press to go into "Process" interface. Input the values of "Begin Time", "End Time" and "Factor" separately and press to confirm. Then the value of "I.U." would display on the screen.

Step 7. Search Peaks

After scan finished, press to go into search mode. Press , to search the value of every point.

13.5.DNA/Protein Mode

Step 1. Enter into DNA/Protein Mode

Main menu, press or , to choose "DNA/Protein Mode" and press to confirm.

Step 2. Setup Parameters

Press to set coefficients, input all the values of f1 to f4 by numeric keypad according the indication and press to confirm.

Step 3. Choose Measure Method

Press to set method. Press , to choose "Absorbance Difference 1" or "Absorbance Difference 2" followed with pressed to confirm. If users don't want to measure reference, use , to choose "No" followed with pressed to confirm the choice.

Step 4. Set Concentration Unit

Press to set concentration unit. Use , to select unit and press to confirm.

Step 5. Measure Samples

Put the sample to be measured in the main light path and put Reference i