Operating Manual for BSDBU-301

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1.Safety
2.Package Contents
3.Unpacking
4.Installation
    4.1. Environment Required
    4.2. Install Spectrophotometer
5.Overview
6.Symbols
7.Main Specifications
8.Description of Appearance and Keys
    8.1.Appearance
    8.2.Keypad
    8.3.Description of Keys
9.Functions
    9.1.Photometry
    9.2.Quantitation
    9.3.Wavelength Scan
    9.4.Kinetics
    9.5.DNA/Protein Measurement
    9.6.Multi-wavelength
10.Getting Started
11.Important Guidelines
12.General Operating
13.Measuring
    13.1.Photometry
    13.2.Quantitation
    13.3.Wavelength Scan
    13.4.Kinetics
    13.5.DNA/Protein Mode
    13.6.Multi Wavelength Mode
    13.7.System Utility
14.Troubleshooting
15.Repair and Maintenance
    15.1.Daily Maintain
    15.2.Spare Parts Replacement
16.Warranty
17.Equipment Disposal

1.Safety

Please follow the guidelines below, and read this manual in its entirety to ensure safe operation of the unit.
We recommends against the use of BSDBU-301 Spectrophotometer.

Do not open the device.
Disconnect the device from the mains supply before carrying out maintenance work or changing the fuses.
The inside of the device is a high-voltage area Danger!
Do not use the device if it is damaged, especially if the main power cable is in any way damaged or defective.
Repairs may only be carried out by the service technicians from us and authorized contractual partners.
The device must be connected to a power outlet that has a protective ground connection.
If the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired.

Do not allow any liquid to enter into the device.
Do not operate the device in a hazardous location or potentially explosive environment.

2.Package Contents

Description	Quantity
Spectrophotometer	1PC
10mm Glass Cuvette	4PCS
10mm Quartz Cuvette	2PCS
Power Cord	1PC
User’s Manual	1PC
Dust Cover	1PC

3.Unpacking

Open the package, according to carefully check the packaging packing list items, if found inside the packaging are missing or damaged items please

contact us and authorized contractual partners.

4.Installation

4.1. Environment Required

To ensure the best performance, the following conditions are required:

The best working temperature range is 16—35℃ and the humidity is 45—80%.

Keep it as far as possible away from the strong magnetic or electrical fields or any electrical device that may generate high-frequency fields.

Set the unit up in an area that is free of dust, corrosive gases and strong vibrations.

Remove any obstructions or materials that could hinder the flow of air under and around the instrument.

The power requirement is 110±11V/60±1Hz or 220±22V/50±1Hz.

Use the appropriate power cord and plug into a grounded outlet.

If the local voltage is not stable, a voltage regulator is required.

Be away from direct sunlight.

4.2. Install Spectrophotometer

Placement

Place the instrument on the stable table carefully.

Install Printer (Printer is Optional Accessories)

Check to confirm instrument power switch is turned off, connect the printer’s data cable to the instrument’s parallel port.

Link the Power Cord

Check to confirm instrument power switch is turned off, the power cord plug into two separate power interface and power supply socket apparatus.

5.Overview

BSDBU-301 Spectrophotometer is an electrical measure instrument which is widely used in the laboratories.

Use Frequency: Intermittence

Excessive Voltage(Current): No

Pollution Class: Class 1

6.Symbols

The following chart is an illustrated glossary of the symbols that are used in this manual.

Caution, Danger!

Caution, High Voltage!

Caution, Hot!

Ground

Fuse

Recycle, this instrument will be called back by the appointed Electrical Treatment Department or by the original Manufacturer when wasted.

7.Main Specifications

Optical System Double beam

Wavelength Range 190—1100nm

Wavelength Accuracy ±0.3nm

Wavelength Repeatability 0.2nm

Photometric Range -0.3—3A, 0—200%T

Photometric Accuracy ±0.3%T

Photometric Repeatability 0.2%T

Spectral Bandwidth 1.8nm

Stray Light 0.05%T@220nm&360nm

Stability ±0.001A/h@500nm

Work Mode
Photometry, Quantitation, Kinetics, Wavelength Scan, DNA/Protein, Multi Wavelength

Interface USB, Parallel(printer)

Power Requirement AC 110/220V, 50/60Hz

Dimensions 600x450x260

Weight 20kg

Work Environment 15 — 35 ℃ , 15 — 70% relative humidity

Store Environment -10 — 50 ℃ , 15 — 70% relative humidity

8.Description of Appearance and Keys

8.1.Appearance

Front View



Back view


1 LCD Display Lid of Sample Room
2 Sample holder
3 LCD Display
4 Keypad
5 USB Port(Type A, USB Memory)
6 Cover of Fan
7 Power Switch
8 Power Socket
9 LCD Contrast Adjust
10 Printer port
11 USB port (Type B, Communication)

8.2.Keypad

8.3.Description of Keys

Function Keys: Functions on-screen prompts



… Numeric Keys: Enter numbers and letters

CLEAR Key: Delete the input value or stored data

ESC Key: Return to previous Interface

100%T/0Abs Key: Blank

OPEN Key: Open files stored in internal memory

SAVE Key: Save files to internal memory

START/STOP Key: Start/Stop testing

GOTO ʎ Key: Set wavelength

PRINT Key: Print measuring result

ENTER Key: Confirm operation

CELL Key: Select/Deselect Auto-cell Holder

RIGHT, LEFT Keys: Search peak/valley and set X scale

UP, DOWN Keys: Scroll menu/data and set Y scale

9.Functions

9.1.Photometry

Display results as Abs, %T or Concentration.

9.2.Quantitation

We provide 2 methods to establish a Standard Curve:
2 methods to set up the regression curve.
Up to 10 standard samples to calibrate the regression curve.

3 methods for curve fit.
Linear fit, Quadratic fit and Cubic fit.

9.3.Wavelength Scan

Allow user to set scan step (0.1, 0.2, 0.5, 1.0 and 5.0nm).

Spectrum display mode can be changed (Wavelength-%Transmittance or Wavelength-Absorbance).

9.4.Kinetics

Allow user to set scan Interval (0.5, 1.0, 2.0, 5.0, 10, 30 and 60s). Spectrum display mode can be changed (Time-%Transmittance or Time-Absorbance).

9.5.DNA/Protein Measurement

Wavelength points and ratios can be set up. Results will be grouped into a table format automatically.

9.6.Multi-wavelength

Up to 10 wavelength points can be set up. Results will be grouped into a table format automatically.

10.Getting Started

The following chart describes the basic operation of the instrument.

Turn On and Self-check

Switch on the power. Then the instrument begins to self-check and 15 minutes’ warm up.
Self-check includes the following steps:
Turn on lamps → Check Sensor → Initialize AD → System position → Get Dark Current → Warm up.



Finished self-check, “System calibration” interface displays, press the keys , to select to do calibration or not.


After system do or skip calibration, instrument displays Main Interface.

11.Important Guidelines

Reagents and dilution buffers can cause cauterization and other damage to health.

Samples (nucleic acids, proteins, bacteria cultures) can be infectious and cause serious damage to health.

During sample preparation, measuring procedures and maintenance and cleaning work, observe all local laboratory safety precautions (e.g. wear protective clothing and gloves, use of disinfectant) regarding the handling of sample material.

Dispose of measuring solutions and cleaning and disinfectant materials in accordance with the relevant local laboratory regulations.

12.General Operating

Select Application

Main menu, press numeric keys or use the key , to choose corresponding menu, then press to enter into.

Set Wavelength
Press to set wavelength, use numeric keys to input the values, press to confirm and go to the point you set, then do blank automatically.

Set Parameters
Press function key to set parameters, press , to choose or input the values by numeric keys, press to enter into, press to return.

Set Auto-cell Holder(Optional) Press to active the auto cell holder and press the numeric key (1-8)to make corresponding cell position at the light path. Press again to inactive the auto cell holder.

Delete the Input Value
Press to delete a character, press to delete all the characters.

Delete the Test Results and Stored data
Press to delete the test result or stored data.

Blank
Put the Reference in the light path, press to do blank.

Measure Samples
Put the samples in the light path, press to measure.

Print the Test Results

Store the Test Results
Press to store the test results, input the file name by numeric keys
and press to save. If the USB Disk was installed, the data will save
in the USB Disk, or the data will save in the internal memory.
Load the Stored File

In the test interface, press to go into file selecting interface,
press , to choose the file you want, press to open. If the USB Disk was installed, the data will open from the USB Disk, or the data will from the internal memory.

13.Measuring

13.1.Photometry

Step 1. Start Photometry

Main menu, press numeric key Or , to choose “Basic Mode”, then press


.

Step 2.Set Photometric Mode
Press to set photometric mode. Press , to choose “Abs.”, “T%” or “Conc./Factor” and press to confirm. If users choose “Abs.” or “T%”, please go to step 5 directly.

Step 3.Set Concentration Unit
Press to set concentration unit. Press , to choose unit followed with pressed to confirm. You can also choose “other” to input the unit.

Step 4. Set “Factor” or “Standard”
Two methods are under your choice:

Method 1 ： Input Factor F

Press to set F. Input the value of F by numeric keypad, press to confirm. Then the F value would display on the screen.

Method 2 ： Use Standard Sample

Put the reference sample in the light path and calibrate 100%T/0Abs; Put the standard sample in the light path ， press to start the mark. Input the concentration value of the standard and press to confirm, then it displays on the screen.

Step 5.Set Wavelength
Press to set wavelength, input the value by the numeric keypad
followed with Pressed to confirm.

Step 6. Measure samples
Put the sample to be measured in the main light path and put Reference in the reference light path, then the result displays on the screen automatically.

13.2.Quantitation

Step 1. Start Quantitation

Main menu, press or press , to choose “Quantitative Mode”
followed with Pressed to confirm.


Step 2. Set Unit
Press to set concentration unit, press , to choose and press to confirm.

Step 3.Establish Standard Curve or load the stored curve

Press to go into set up interface, 2 methods are under your choice.



Establish Standard Curve:

Method 1: Input Regression Equation
1) Set Fit Curve Method. Press to set Fit method，use ,
to choose the method and press to confirm.

2) Set Wavelength. Press to set wavelength. Use , to choose measure method, then press to confirm. Input the wavelength value you need and press to confirm.

3) Setup Standard Samples. Press to setup standard, input the
concentrations of corresponding standard samples according the
indication and press to confirm. Users can use , to
choose the value you just input and press to delete, then
input a new value, press to confirm. Press to cancel
after all the input.


4) Calibrate Standard Samples. Put the corresponding standard samples in the main light path and put Reference in the reference light path as the screen indicates and press to measure. Then the Abs. value would appear in the corresponding table.Load the Stored CurvesIn the “Calibration Table”interface, press to go into files select interface. Use , to select the curve you need and press to load.



Load the Stored Curves
In the “Calibration Table” interface, press to go into files select interface. Use , to select the curve you need and press to load. Users can press to view the curve.


Step 4. Return the sample measurement interfaceIn the “Calibration Table” interface, press to return the Quantitative Test interface.



Step 5. Measure Samples
Place the sample to be tested in the light path, press to measure.Then the test result will display in the data sheet. Repeat this step to finish measuring all the samples.

13.3.Wavelength Scan

Step 1. Enter into Wavelength Scan
Main menu, press numeric key or , to choose “Wavelength Scan” and press to enter.

Step 2. Parameters Setup
Press to set parameters, set “Scan From” , “scan to”, “scan step”and “scan speed”, press to confirm.

Step 3. Set Photometric Mode
Press to set photometric mode，choose “T%”, “Abs.” or “E” and press to confirm.

Step 4. Scan Samples
Put the sample to be measured in the main light path and put Reference in the reference light path, press to scan the sample, press to cancel.


Step 5. Search Peaks
After scanned, press to go into peak search mode. Press to set peak height, input the peak height and press to confirm.Press , to display the value of every wavelength point. Press , to display the value of every peak.



Step 6. Smooth the Curve
After scanned, if there are many burrs on it, press to smooth the curve.

13.4.Kinetics

Step 1. Enter into Kinetics
Main menu, press or , to select “Kinetics Mode” and press to confirm.


Step 2. Setup Parameters
Press to set parameters, input the corresponding values of“Total Time”, “Delay Time” and “Time Intervals” according the screen indicates. Press to confirm.

Step 3. Set Photometric Mode
Press to set photometric mode, choose “T%” or “Abs.” and press to confirm.

Step 4. Set Wavelength
Press to to set wavelength, input the value of the wavelength by numeric keypad and press to confirm

Step 5. Measure Samples
Put the sample to be measured in the main light path and put Reference in the reference light path, press to begin the test, press to cancel.



Step 6. Calculate Response Rate
After time scanned, if users want to calculate the response rate of some period, you can press to go into “Process” interface. Input the values of “Begin Time”, “End Time” and “Factor” separately and press to confirm. Then the value of “I.U.” would display on the screen.

Step 7. Search Peaks
After scan finished, press to go into search mode. Press , to search the value of every point.

13.5.DNA/Protein Mode

Step 1. Enter into DNA/Protein Mode
Main menu, press or , to choose “DNA/Protein Mode” and press to confirm.


Step 2. Setup Parameters
Press to set coefficients, input all the values of f1 to f4 by numeric keypad according the indication and press to confirm.

Step 3. Choose Measure Method
Press to set method. Press , to choose “Absorbance Difference 1” or “Absorbance Difference 2” followed with pressed to confirm. If users don’t want to measure reference, use , to choose “No” followed with pressed to confirm the choice.

Step 4. Set Concentration Unit
Press to set concentration unit. Use , to select unit and press to confirm.

Step 5. Measure Samples
Put the sample to be measured in the main light path and put Reference in the reference light path, press to measure. The result will display in the data sheet.

13.6.Multi Wavelength Mode

Step 1. Enter into Multi Wavelength Measurement

Main Menu, press or , to choose “Multi Wavelength Measurement” and then press to go into multi wavelength measurement interface.


Step 2. Setup Wavelength

Press to go into wavelength setting interface，input all the wavelength value one by one by the numeric keypad. Press to confirm, press to return.

Step 3. Set Photometric Mode
Press to set the photometric mode， use , to select “Abs. ” or “T%” mode, press to confirm.

Step 4. Measure Samples
Put the sample to be measured in the main light path and put Reference in the reference light path, press to measure, the test result will display in the data table.

13.7.System Utility

Main menu, press or use , to select “System Utility” and press to confirm.

13.7.1 Wavelength Reset

Press or use , to choose “Wavelength Reset” then press to begin the calibration. During the course, opening the lid of the compartment is prohibited.

13.7.2.Printer Setup

Press or , to select “Printer Setup” then press to confirm.



Reset Printer
Press or use , to choose “Reset Printer” and press to confirm. Then the printer will resume the initial condition.

Select print port
Press or use , to choose “Select print port” and press to confirm. Use , to choose “LPT” or “Comm.” and then press to confirm.

Select Printer
Press or use , to choose “Select printer” and press to confirm. Use , to select the printer’s model and then press to confirm.

Change Print Mode
Press or use , to choose “Change print mode” and press
to confirm. Two modes are under your choice, one is “Print Data
Sheet”, the other is “print the display interface”.

13.7.3.Lamp Service

Press or use , to choose “Lamp Service”, press to go into “lamps management” interface.



Switch On/Off D2 lamp
Press or , to choose “Switch On/Off D2 lamp” then press to switch on or switch off the D2 lamp.

Reset the usage time of D2 lamp
Press or use , to choose “Reset D2 lamp usage time” and
press . Then users can find the D2 lamp’s usage time and the system
will ask if you are sure to reset the usage time. Press , to choose “Yes” and then press to confirm. The system will record the usage time from zero.

Switch On/Off W lamp
Press or use , to choose “Reset W lamp usage time” and
press . Then users can find the W lamp’s usage time and the system
will ask you if are sure to reset the usage time.

Reset the W lamp usage time
Press or use , to choose “Reset W lamp usage time” and press . Then users can find the W lamp’s usage time and the system will ask if you are sure to reset the usage time. Press , to choose “Yes” and then press to confirm. The system will record the usage time from zero.

Change Switch point
Press or use , to choose “Change Switch point” and press to confirm. Input the wavelength point value(325—375nm)and press to confirm.

13.7.4.Clock Setup

Press or use , to choose “Clock Setup”, then press to go
into “Clock Management” Interface.



Set Time

Press or , to choose “Set Time” and press to confirm.Input the time (Hour, Minute, Second) by numeric keypad, press to confirm and return automatically.

Set Date
Press or use , to choose “Set Date” and press to confirm. Input the Date(Year, Month, Day) by numeric keypad, press to confirm and return automatically.

Display Time
Press or use , to choose “Display Time” and press to confirm. Then the time will display on the Right Top corner.

Display Date
Press or use , to choose “Display Date” and press to confirm. Then the Date will display on the Right Top corner.

13.7.5.Refresh Dark Curren

Press or use , to choose “Refresh Dark Current” and press to confirm. Then the system begins to refresh Dark Current.

Note: During the course, open the lid of the compartment is prohibited.

13.7.6.Connect to PC

Press or use , to choose “Connect to PC”, and press to go into waiting interface. When the instrument was connected to PC, it displays “Controlled by PC”.

13.7.7.Beeper on/off

Press or use , to choose “Beeper on/off” and press to switch on or switch off the beeper.

13.7.8.Refresh System Baseline

Press or use , to choose “Refresh System Baseline” and press to confirm. Then the system will scan the baseline. During this course, opening the lid of the compartment is prohibited

13.7.9.Deleted Entire Saved Files

Use , to choose “Deleted Entire Saved Files” and press to confirm. Then the system ask you “Delete entire files, are you sure?”, use , to choose “Yes” , then all the files in the RAM will be deleted.

13.7.10.Restore Default Settings

Use , to choose “Restore Default Settings” and press to Confirm, then the system will restore the initialization.

14.Troubleshooting

Review the information in the table below to troubleshoot operating problems.

Problem	Cause	Solution
Power on, no response	Power cord connection is not reliable	Improve connectivity
	Fuse burning	Replace fuse
Measurement uncertainty	Warm up is not enough	Warm up more time
	Glass cuvettes used in UV Range	Use quartz cuvettes
	Sample is not Stable	Improve the sample
	The concentration of sample is too high	Diluted sample
	Power Supply Voltage Low or not Stable	Improve the Power Supply
	Lamp damage or lamp life maturity	Replace lamp
Dark Current Error when self-check	The lid of the compartment is open during self-check	Close the lid, restart
System Calibrate Failed	Something block the Light path	Remove it,calibrate again
Power on, back light is OK, but nothing display on the screen or display is not clear	Display Contrast problem	Adjust the contrast potentiometer
Measurements inaccurate	Cuvettes were contaminated	Clean cuvettes
	Samples were contaminated	Improve samples
	Worse matching of the cuvettes	Improve the matching of the cuvettes
	Dark current error	Resample dark current

15.Repair and Maintenance

15.1.Daily Maintain

Check the Compartment
After measurement, the cuvettes with sample solutions should be taken out of the compartment in time. Or the volatilization of the solution would make the mirror go moldy. Users must pay more attention to the corrosive sample and liquid easy to volatilize. Any solution remains in the compartment should be wiped off immediately.
Surface Clean
The cover of the instrument is with paint. Please use wet towel to wipe off the drips on the surface immediately. Organic solution is forbidden to be used to clean the cover. Please wipe off the dirt on the cover timely.
Clean the Cuvettes
After every test or after a solution change, the cuvettes should be cleaned carefully, or the remains on the surface would cause measuring error.

15.2.Spare Parts Replacement

Replace the Fuse

Danger! Be sure to switch off the power and unplug the socket before replacement!

Step 1.
Tools preparation
Prepare a 3×75 Flat Blade screwdriver .

Step 2.
Switch Off the power supply
Switch off the power supply, and unplug the socket.

Step 3.
Take out the Fuse Seat
Take out the fuse seat by the screwdriver.


Step 4. Replace a new fuse
Pick out the spare fuse (3.15A/250V) and replace it to the working position.


Step 5. Reset the fuse seat
Replace the fuse seat in the power socket.

Step 6. Switch on the power
Plug the socket and switch on the power.

Replace Lamps

Hot! Wait 20 minutes before open the lamp chamber after power
off to avoid scald!

Step 1. Tools preparation
Prepare a 6×150mm Cross Blade screwdriver and a pair of glove.

Step 2. Power Off
Switch off the power supply and unplug the socket.

Step 3. Open the cover
Unscrew the 4 screws indicated(Each side with 2 screws) and remove the cover.


Step 4. Open the cover of the light chamber
Unscrew the 2 screws on the light chamber cover and remove it


Step 5. Replace the D2 lamp
Unscrew the 2 screws on the D2 Flange (No.1), unplug the connector in the Power Board (No. 2) and remove the D2 lamp. Draw on the cotton glove and replace a new lamp. Fix the 2 screws and plug the connector again.

Step 6. Replace W lamp
Pull out the defected W lamp and draw on the cotton glove. Insert the new W lamp as deep as possible on the lamp seat.


Step 7. Adjust the position of the W lamp
Switch on the power(the Switch Mirror should be placed to the position as indicates). Observe the entrance facular, adjust the W Lamp until the light is in the center of the entrance hole.


Step 8. Finish

Reset the cover of the light chamber and fix the screws. Reset the cover of the instrument and fix the screws. Recover the Pole in the compartment, then the course finished.

Replace the Battery
Danger! Be sure to switch off the power and unplug the socket before replacement!

Step 1. Prepare the tools
Prepare a 6×150mm Cross Blade Screwdriver.

Step 2. Switch off the power supply
Switch off the power supply and unplug the socket.

Step 3. Open the Bottom cover plate
Unscrew all the screws indicated then remove the bottom plate.



Step 4. Replace the Battery
Pick out the old battery and replace a new one.


Step 5. Finish
Recover the bottom plate and fix all the screws, then the course finishes.

16.Warranty

We warrant that this product will be free from defects in material and 30 workmanship for a period of one (1) year from date of purchase. If a defect is present, we will, at its option, repair, replace, or refund the purchase price of this product at no charge to you, provided it is returned during the warranty period. This warranty does not apply if the product has been damaged by accident, abuse, misuse, or misapplication, or from ordinary wear and tear.

For your protection, items being returned must be insured against possible damage or loss. This warranty shall be limited to the replacement of defective products. IT IS EXPRESSLY AGREED THAT THIS WARRANTY WILL BE IN LIEU OF ALL WARRANTIES OF FITNESS AND IN LIEU OF THE WARRANTY OF MERCHANTABILITY.

17.Equipment Disposal

This equipment is marked with the crossed out wheeled bin symbol to indicate that this equipment must not be disposed of with unsorted waste. Instead it's your responsibility to correctly dispose of your equipment at lifecycle -end by handling it over to an authorized facility for separate collection and recycling. It's also your responsibility to decontaminate the equipment in case of biological, chemical and/or radiological contamination, so as to protect from health hazards the persons involved in the disposal and recycling of the equipment.
For more information about where you can drop off your waste of equipment, please contact your local dealer from whom you originally purchased this
equipment.
By doing so, you will help to conserve natural and environmental resources
and you will ensure that your equipment is recycled in a manner that
protects human health.
Thank you！