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Spectrophotometer
Double Beam UV Visible Spectrophotometer
Double Beam UV Visible Spectrophotometer BSDBU-301
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Double Beam UV Visible Spectrophotometer BSDBU-301
- Sea, Air, Door to Door Shipping
- 1 Year Warranty
- US & European Standards
Designed to conserve benchspace, our product offers the measurement range of 190-1100nm at bandwidth of 1.8nm and 2nm. Engineered for a wide range of applications, our systems provide dependable performance and reliable results. It produces the photometric range of -0.3 to 3 A; 0-200 %T and photometric accuracy of ±0.2%T which makes it suitable for various quantitative assays.
- Large LCD display, all data and graphs can be shown directly and clearly
Specification
Features
Applications Optional Accessories
Wavelength Range | 190-1100 nm |
Wavelength Accuracy | ±0.3 nm |
Wavelength Repeatability | 0.2 nm |
Spectral Bandwidth | 1.8 nm |
Photometric Range | 0-200%T, -0.3-3.0 A |
Photometric Accuracy | ±0.3%T or ±0.003A @ 1A |
Stability | 0.0003 A/h @ 500nm |
Baseline Flatness | ±0.0005 A |
Stray Light | 0.04%T @ 220 nm , 360 nm |
Light Source | Tungsten and Deuterium Lamp |
Software | Optional PC model with UV/Vis analyst software |
Gross Dimension (W/D/H) | 589x428x200 mm |
Display | 5 inches LCD (320*240 dots) |
Weight (Net/Gross) | 22 kg |
Power Supply | AC 110/220 V 60 Hz |
- Large LCD display, all data and graphs can be shown directly and clearly
Most suitable for quantitative determination assays and toxicological assays in environmental, industrial, pharmaceutical fields.
Accessory Code | Name | Description | Unit | Light Path (mm) |
3000806006 | Holder | 4-cell holder for 10mm square Cuvette | ||
3000806007 | Holder | 4-cell holder for up to 50mm square cuvette | ||
3000806008 | Holder | 4-cell holder for up to 100mm square cuvette | ||
3000806009 | Holder | Long path single cell holder for 100mm | ||
3000806010 | Holder | Single cell holder for 10mm square Cuvette | ||
3000806011 | Holder | Cylindrical cell holder(Φ16mm) | ||
3000806012 | Holder | Water-jacketed single cell holder | ||
3000806013 | Holder | Test tube holder(Φ8-Φ22mm) | ||
3000806014 | Holder | 3-position cuvette holder | ||
3000806015 | Holder | 3-position cuvette holder | ||
3000806016 | Holder | Solid sample holder(δ1.5mm--3mm;single cell) | /1pc | |
3000806017 | Holder | Solid sample holder(δ1.5mm--3mm;double cell) | /1pc | |
3000806018 | Holder | 10mm water-jacked 4 cell holder | /1pc | |
3000806019 | Other | 5° reflectance meachment attachment (minimum sample:20x20mm ;central beam height:50mm) | /1pc | |
3000806020 | Lamp | 6v10w halogen lamp | /1pc | |
3000806021 | Lamp | 12v20w halogen lamp (phillips) | ||
3000806022 | Lamp | 12v20w halogen lamp (osram) | ||
3000806023 | Lamp | Milas deuterium lamp; 10v;300ma; 30w | ||
3000806024 | Lamp | Deuterium lamp | /1pc | |
3000806025 | Thermo printer | |||
3000806026 | Stylus printer | |||
3000806027 | Cuvettes | Square cuvettes; glass 10mm | /4pcs | |
3000806028 | Cuvettes | Square cuvettes; glass 20mm | /4pcs | |
3000806029 | Cuvettes | Square cuvettes; glass 30mm | /4pcs | |
3000806030 | Cuvettes | Square cuvettes; glass 50mm | /4pcs | |
3000806031 | Cuvettes | Square cuvettes; glass 100mm | /4pcs | |
3000806032 | Cuvettes | Square cuvettes; quartz 10mm | /2pcs | |
3000806033 | Cuvettes | Square cuvettes; quartz 20mm | /2pcs | |
3000806034 | Cuvettes | 500ul micro cell ; central-beam height:8.5mm;15mm; 2x10x45mm; quartz | /1pc | |
3000806035 | Cuvettes | 1000ul micro cell; central-beam height:8.5mm;15mm; 3x10x45mm; quartz | /1pc | |
3000806036 | Software | Dongles for 31.61 application software ; (UV analyst software) | /1set | |
3000806037 | Software | Software (m.wave basic) | /1set | |
3000806038 | Software | Software | /1set | |
3000806039 | Software | Advanced PC window software (m.wave professional) | /1set | |
3000806040 | Software | Software (m.wave basic) | /1set | |
3000806041 | Holder | Cell holder for micro cells; Central beam height: 15mm | ||
3000806042 | USB connector line | USB connector line (only for m.wave basic) | /1pc | |
3000806043 | Quartz Cuvette | Cell; 100ul; 1pcs; central beam height: 15mm | 10 | |
3000806044 | Quartz Cuvette | Cell; 200ul; 1pcs;central beam height:15mm | 10 | |
3000806045 | Cuvette | Flow cell; 5 mm; optical glass; 1pcs;central beam height:15mm; l5xw2xh5mm | ||
3000806046 | Cuvette | Flow cell; 10 mm; optical glass; 1pcs;central beam height:15mm; l10xw2xh5mm | ||
3000806047 | Cuvette | Flow cell; 20 mm; optical glass; 1pcs;central beam height:15mm;l20xw2xh5mm | ||
3000806048 | Cuvette | Flow cell; 30 mm; optical glass; 1pcs;central beam height:15mm; l30xw2xh5mm | ||
3000806049 | Cuvette | Flow cell; 5 mm; quartz; 1pcs; central beam height:15mm; l5xw2xh5mm | ||
3000806050 | Cuvette | Flow cell; 20 mm; quartz; 1pcs; central beam height:15mm; l20xw2xh5mm | ||
3000806051 | Cuvette | Flow cell; 30 mm; quartz; 1pcs; central beam height:15mm; l30xw2xh5mm | ||
3000806052 | Other | A-100 peltier/sipper system; constant-temperature sipper system; holder beam height:15mm | ||
3000806053 | Holder | 1-cell holder for 100mm square cuvette | ||
3000806054 | Holder | 10mm water-jacketed 4-cell holder | ||
3000806055 | Holder | Test tube holder(Φ8-Φ22mm) | ||
3000806056 | Holder | Solid sample holder(δ1.5mm-3mm; 1-position) | ||
3000806057 | Silicon tube | Silicon tube; 1bag=1pair(1m+1m) | /1bag | |
3000806058 | Glass cuvettes | Square cuvettes; volume:1800ul | /2pcs | 5 |
3000806059 | Glass cuvettes | Square cuvettes; volume:1350ul | 3 | |
3000806060 | Glass cuvettes | Square cuvettes; volume:900ul | 2 | |
3000806061 | Glass cuvettes | Square cuvettes; volume:450ul | 1 | |
3000806062 | Quartz cuvettes | Square cuvettes; volume:1800ul | /2pcs | 5 |
Operating Manual for BSDBU-301
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1.Safety
2.Package Contents
3.Unpacking
4.Installation
4.1. Environment Required
4.2. Install Spectrophotometer
5.Overview
6.Symbols
7.Main Specifications
8.Description of Appearance and Keys
8.1.Appearance
8.2.Keypad
8.3.Description of Keys
9.Functions
9.1.Photometry
9.2.Quantitation
9.3.Wavelength Scan
9.4.Kinetics
9.5.DNA/Protein Measurement
9.6.Multi-wavelength
10.Getting Started
11.Important Guidelines
12.General Operating
13.Measuring
13.1.Photometry
13.2.Quantitation
13.3.Wavelength Scan
13.4.Kinetics
13.5.DNA/Protein Mode
13.6.Multi Wavelength Mode
13.7.System Utility
14.Troubleshooting
15.Repair and Maintenance
15.1.Daily Maintain
15.2.Spare Parts Replacement
16.Warranty
17.Equipment Disposal
1.Safety
Please follow the guidelines below, and read this manual in its entirety to ensure safe operation of the unit.We recommends against the use of BSDBU-301 Spectrophotometer.
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Disconnect the device from the mains supply before carrying out maintenance work or changing the fuses.
The inside of the device is a high-voltage area Danger!
Do not use the device if it is damaged, especially if the main power cable is in any way damaged or defective.
Repairs may only be carried out by the service technicians from us and authorized contractual partners.
The device must be connected to a power outlet that has a protective ground connection.
If the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired.
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Do not operate the device in a hazardous location or potentially explosive environment.
2.Package Contents
Description | Quantity |
Spectrophotometer | 1PC |
10mm Glass Cuvette | 4PCS |
10mm Quartz Cuvette | 2PCS |
Power Cord | 1PC |
User’s Manual | 1PC |
Dust Cover | 1PC |
3.Unpacking
Open the package, according to carefully check the packaging packing list items, if found inside the packaging are missing or damaged items pleasecontact us and authorized contractual partners.
4.Installation
4.1. Environment Required
To ensure the best performance, the following conditions are required:
The best working temperature range is 16—35℃ and the humidity is 45—80%.
Keep it as far as possible away from the strong magnetic or electrical fields or any electrical device that may generate high-frequency fields.
Set the unit up in an area that is free of dust, corrosive gases and strong vibrations.
Remove any obstructions or materials that could hinder the flow of air under and around the instrument.
The power requirement is 110±11V/60±1Hz or 220±22V/50±1Hz.
Use the appropriate power cord and plug into a grounded outlet.
If the local voltage is not stable, a voltage regulator is required.
Be away from direct sunlight.
4.2. Install Spectrophotometer
Placement
Place the instrument on the stable table carefully.
Install Printer (Printer is Optional Accessories)
Check to confirm instrument power switch is turned off, connect the printer’s data cable to the instrument’s parallel port.
Link the Power Cord
Check to confirm instrument power switch is turned off, the power cord plug into two separate power interface and power supply socket apparatus.
5.Overview
BSDBU-301 Spectrophotometer is an electrical measure instrument which is widely used in the laboratories.Use Frequency: Intermittence
Excessive Voltage(Current): No
Pollution Class: Class 1
6.Symbols
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7.Main Specifications
Optical System Double beamWavelength Range 190—1100nm
Wavelength Accuracy ±0.3nm
Wavelength Repeatability 0.2nm
Photometric Range -0.3—3A, 0—200%T
Photometric Accuracy ±0.3%T
Photometric Repeatability 0.2%T
Spectral Bandwidth 1.8nm
Stray Light 0.05%T@220nm&360nm
Stability ±0.001A/h@500nm
Work Mode
Photometry, Quantitation, Kinetics, Wavelength Scan, DNA/Protein, Multi Wavelength
Interface USB, Parallel(printer)
Power Requirement AC 110/220V, 50/60Hz
Dimensions 600x450x260
Weight 20kg
Work Environment 15 — 35 ℃ , 15 — 70% relative humidity
Store Environment -10 — 50 ℃ , 15 — 70% relative humidity
8.Description of Appearance and Keys
8.1.Appearance
Front View
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Back view
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1 LCD Display Lid of Sample Room
2 Sample holder
3 LCD Display
4 Keypad
5 USB Port(Type A, USB Memory)
6 Cover of Fan
7 Power Switch
8 Power Socket
9 LCD Contrast Adjust
10 Printer port
11 USB port (Type B, Communication)
8.2.Keypad
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8.3.Description of Keys
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…
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9.Functions
9.1.Photometry
Display results as Abs, %T or Concentration.9.2.Quantitation
We provide 2 methods to establish a Standard Curve:2 methods to set up the regression curve.
Up to 10 standard samples to calibrate the regression curve.
3 methods for curve fit.
Linear fit, Quadratic fit and Cubic fit.
9.3.Wavelength Scan
Allow user to set scan step (0.1, 0.2, 0.5, 1.0 and 5.0nm).Spectrum display mode can be changed (Wavelength-%Transmittance or Wavelength-Absorbance).
9.4.Kinetics
Allow user to set scan Interval (0.5, 1.0, 2.0, 5.0, 10, 30 and 60s). Spectrum display mode can be changed (Time-%Transmittance or Time-Absorbance).9.5.DNA/Protein Measurement
Wavelength points and ratios can be set up. Results will be grouped into a table format automatically.9.6.Multi-wavelength
Up to 10 wavelength points can be set up. Results will be grouped into a table format automatically.10.Getting Started
The following chart describes the basic operation of the instrument.Turn On and Self-check
Switch on the power. Then the instrument begins to self-check and 15 minutes’ warm up.
Self-check includes the following steps:
Turn on lamps → Check Sensor → Initialize AD → System position → Get Dark Current → Warm up.
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Finished self-check, “System calibration” interface displays, press the keys
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After system do or skip calibration, instrument displays Main Interface.
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11.Important Guidelines
Reagents and dilution buffers can cause cauterization and other damage to health.Samples (nucleic acids, proteins, bacteria cultures) can be infectious and cause serious damage to health.
During sample preparation, measuring procedures and maintenance and cleaning work, observe all local laboratory safety precautions (e.g. wear protective clothing and gloves, use of disinfectant) regarding the handling of sample material.
Dispose of measuring solutions and cleaning and disinfectant materials in accordance with the relevant local laboratory regulations.
12.General Operating
Select ApplicationMain menu, press numeric keys or use the key
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Set Wavelength
Press
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Set Parameters
Press function key to set parameters, press
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Set Auto-cell Holder(Optional) Press
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Delete the Input Value
Press
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Delete the Test Results and Stored data
Press
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Blank
Put the Reference in the light path, press
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Measure Samples
Put the samples in the light path, press
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Print the Test Results
Store the Test Results
Press
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and press
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in the USB Disk, or the data will save in the internal memory.
Load the Stored File
In the test interface, press
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press
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13.Measuring
13.1.Photometry
Step 1. Start PhotometryMain menu, press numeric key
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Step 2.Set Photometric Mode
Press
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Step 3.Set Concentration Unit
Press
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Step 4. Set “Factor” or “Standard”
Two methods are under your choice:
Method 1 : Input Factor F
Press
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Method 2 : Use Standard Sample
Put the reference sample in the light path and calibrate 100%T/0Abs; Put the standard sample in the light path , press to start the mark. Input the concentration value of the standard and press
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Step 5.Set Wavelength
Press
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followed with Pressed
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Step 6. Measure samples
Put the sample to be measured in the main light path and put Reference in the reference light path, then the result displays on the screen automatically.
13.2.Quantitation
Step 1. Start QuantitationMain menu, press
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followed with Pressed
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Step 2. Set Unit
Press
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Step 3.Establish Standard Curve or load the stored curve
Press
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Establish Standard Curve:
Method 1: Input Regression Equation
1) Set Fit Curve Method. Press
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to choose the method and press
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2) Set Wavelength. Press
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3) Setup Standard Samples. Press
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concentrations of corresponding standard samples according the
indication and press
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choose the value you just input and press
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input a new value, press
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after all the input.
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4) Calibrate Standard Samples. Put the corresponding standard samples in the main light path and put Reference in the reference light path as the screen indicates and press to measure. Then the Abs. value would appear in the corresponding table.Load the Stored CurvesIn the “Calibration Table”interface, press
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Load the Stored Curves
In the “Calibration Table” interface, press
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Step 4. Return the sample measurement interfaceIn the “Calibration Table” interface, press
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Step 5. Measure Samples
Place the sample to be tested in the light path, press
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13.3.Wavelength Scan
Step 1. Enter into Wavelength Scan
Main menu, press
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Step 2. Parameters Setup
Press
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Step 3. Set Photometric Mode
Press
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Step 4. Scan Samples
Put the sample to be measured in the main light path and put Reference in the reference light path, press
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Step 5. Search Peaks
After scanned, press
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Step 6. Smooth the Curve
After scanned, if there are many burrs on it, press
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13.4.Kinetics
Step 1. Enter into Kinetics
Main menu, press
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Step 2. Setup Parameters
Press
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Step 3. Set Photometric Mode
Press
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Step 4. Set Wavelength
Press to
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Step 5. Measure Samples
Put the sample to be measured in the main light path and put Reference in the reference light path, press
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Step 6. Calculate Response Rate
After time scanned, if users want to calculate the response rate of some period, you can press
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Step 7. Search Peaks
After scan finished, press
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13.5.DNA/Protein Mode
Step 1. Enter into DNA/Protein ModeMain menu, press
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Step 2. Setup Parameters
Press
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Step 3. Choose Measure Method
Press
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Step 4. Set Concentration Unit
Press
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Step 5. Measure Samples
Put the sample to be measured in the main light path and put Reference in the reference light path, press
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13.6.Multi Wavelength Mode
Step 1. Enter into Multi Wavelength Measurement
Main Menu, press
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Step 2. Setup Wavelength
Press
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Step 3. Set Photometric Mode
Press
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Step 4. Measure Samples
Put the sample to be measured in the main light path and put Reference in the reference light path, press
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13.7.System Utility
Main menu, press
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13.7.1 Wavelength Reset
Press
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13.7.2.Printer Setup
Press
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Reset Printer
Press
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Select print port
Press
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Select Printer
Press
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Change Print Mode
Press
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to confirm. Two modes are under your choice, one is “Print Data
Sheet”, the other is “print the display interface”.
13.7.3.Lamp Service
Press
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Switch On/Off D2 lamp
Press
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Reset the usage time of D2 lamp
Press
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press
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will ask if you are sure to reset the usage time. Press
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Switch On/Off W lamp
Press
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press
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will ask you if are sure to reset the usage time.
Reset the W lamp usage time
Press
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Change Switch point
Press
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13.7.4.Clock Setup
Press
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into “Clock Management” Interface.
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Set Time
Press
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Set Date
Press
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Display Time
Press
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Display Date
Press
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13.7.5.Refresh Dark Curren
Press
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Note: During the course, open the lid of the compartment is prohibited.
13.7.6.Connect to PC
Press
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13.7.7.Beeper on/off
Press
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13.7.8.Refresh System Baseline
Press
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13.7.9.Deleted Entire Saved Files
Use
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13.7.10.Restore Default Settings
Use
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14.Troubleshooting
Review the information in the table below to troubleshoot operating problems.Problem | Cause | Solution |
Power on, no response | Power cord connection is not reliable | Improve connectivity |
Fuse burning | Replace fuse | |
Measurement uncertainty | Warm up is not enough | Warm up more time |
Glass cuvettes used in UV Range | Use quartz cuvettes | |
Sample is not Stable | Improve the sample | |
The concentration of sample is too high | Diluted sample | |
Power Supply Voltage Low or not Stable | Improve the Power Supply | |
Lamp damage or lamp life maturity | Replace lamp | |
Dark Current Error when self-check | The lid of the compartment is open during self-check | Close the lid, restart |
System Calibrate Failed | Something block the Light path | Remove it,calibrate again |
Power on, back light is OK, but nothing display on the screen or display is not clear | Display Contrast problem | Adjust the contrast potentiometer |
Measurements inaccurate | Cuvettes were contaminated | Clean cuvettes |
Samples were contaminated | Improve samples | |
Worse matching of the cuvettes | Improve the matching of the cuvettes | |
Dark current error | Resample dark current |
15.Repair and Maintenance
15.1.Daily Maintain
Check the CompartmentAfter measurement, the cuvettes with sample solutions should be taken out of the compartment in time. Or the volatilization of the solution would make the mirror go moldy. Users must pay more attention to the corrosive sample and liquid easy to volatilize. Any solution remains in the compartment should be wiped off immediately.
Surface Clean
The cover of the instrument is with paint. Please use wet towel to wipe off the drips on the surface immediately. Organic solution is forbidden to be used to clean the cover. Please wipe off the dirt on the cover timely.
Clean the Cuvettes
After every test or after a solution change, the cuvettes should be cleaned carefully, or the remains on the surface would cause measuring error.
15.2.Spare Parts Replacement
Replace the Fuse
Step 1.
Tools preparation
Prepare a 3×75 Flat Blade screwdriver .
Step 2.
Switch Off the power supply
Switch off the power supply, and unplug the socket.
Step 3.
Take out the Fuse Seat
Take out the fuse seat by the screwdriver.
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Step 4. Replace a new fuse
Pick out the spare fuse (3.15A/250V) and replace it to the working position.
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Step 5. Reset the fuse seat
Replace the fuse seat in the power socket.
Step 6. Switch on the power
Plug the socket and switch on the power.
Replace Lamps
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off to avoid scald!
Step 1. Tools preparation
Prepare a 6×150mm Cross Blade screwdriver and a pair of glove.
Step 2. Power Off
Switch off the power supply and unplug the socket.
Step 3. Open the cover
Unscrew the 4 screws indicated(Each side with 2 screws) and remove the cover.
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Step 4. Open the cover of the light chamber
Unscrew the 2 screws on the light chamber cover and remove it
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Step 5. Replace the D2 lamp
Unscrew the 2 screws on the D2 Flange (No.1), unplug the connector in the Power Board (No. 2) and remove the D2 lamp. Draw on the cotton glove and replace a new lamp. Fix the 2 screws and plug the connector again.
Step 6. Replace W lamp
Pull out the defected W lamp and draw on the cotton glove. Insert the new W lamp as deep as possible on the lamp seat.
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Step 7. Adjust the position of the W lamp
Switch on the power(the Switch Mirror should be placed to the position as indicates). Observe the entrance facular, adjust the W Lamp until the light is in the center of the entrance hole.
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Step 8. Finish
Reset the cover of the light chamber and fix the screws. Reset the cover of the instrument and fix the screws. Recover the Pole in the compartment, then the course finished.
Replace the Battery
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Step 1. Prepare the tools
Prepare a 6×150mm Cross Blade Screwdriver.
Step 2. Switch off the power supply
Switch off the power supply and unplug the socket.
Step 3. Open the Bottom cover plate
Unscrew all the screws indicated then remove the bottom plate.
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Step 4. Replace the Battery
Pick out the old battery and replace a new one.
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Step 5. Finish
Recover the bottom plate and fix all the screws, then the course finishes.
16.Warranty
We warrant that this product will be free from defects in material and 30 workmanship for a period of one (1) year from date of purchase. If a defect is present, we will, at its option, repair, replace, or refund the purchase price of this product at no charge to you, provided it is returned during the warranty period. This warranty does not apply if the product has been damaged by accident, abuse, misuse, or misapplication, or from ordinary wear and tear.For your protection, items being returned must be insured against possible damage or loss. This warranty shall be limited to the replacement of defective products. IT IS EXPRESSLY AGREED THAT THIS WARRANTY WILL BE IN LIEU OF ALL WARRANTIES OF FITNESS AND IN LIEU OF THE WARRANTY OF MERCHANTABILITY.
17.Equipment Disposal
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This equipment is marked with the crossed out wheeled bin symbol to indicate that this equipment must not be disposed of with unsorted waste. Instead it's your responsibility to correctly dispose of your equipment at lifecycle -end by handling it over to an authorized facility for separate collection and recycling. It's also your responsibility to decontaminate the equipment in case of biological, chemical and/or radiological contamination, so as to protect from health hazards the persons involved in the disposal and recycling of the equipment.
For more information about where you can drop off your waste of equipment, please contact your local dealer from whom you originally purchased this
equipment.
By doing so, you will help to conserve natural and environmental resources
and you will ensure that your equipment is recycled in a manner that
protects human health.
Thank you!