Class II Biosafety Cabinet BCBS-1205

1. Precautions

Under all conditions marked with , it is necessary to consult the document, so as to clarity the nature of potential risks and any countermeasures that must be taken.

Actions or operations which are prohibited.

Actions or operations which must be followed.

Make sure input voltage is correct and stable. The rated load of main power socket should be higher than cabinet consumption. Plug must be well grounded.

In order to avoid air turbulence, the operator should slightly move his arms during experiment. Hands should stay inside the working area at least 1 minute before operating. In order to decrease the times of arms moving into and out of the working area, prepare all the necessary items inside the cabinet before starting experiment.

Moving principles of different samples inside cabinet: When two or more samples need to be moved, be sure that low-polluting samples move to high-polluting samples. Movement of items should also follow the principles of slow-moving.

Samples placed in parallel: Samples should be placed in the cabinet parallel to avoid cross-contamination between samples and blocking back air grille.

In order to avoid samples being sucked into the negative passage or the blower, do not place soft and slight samples (for example: soft tissue) on the surface during experiment;

The weight of items placed in the cabinet should be no more than 23kg/(25x25cm2);

Avoid vibration: avoid using vibration equipment (e.g. centrifuges, vortex oscillator, etc.) inside the cabinet. Vibration would cause lower cleanliness of operating area and affect operator protection.

No flame: No flame is allowed inside the cabinet. Using of fire will lead to airflow disorder, and filter damage. If sterilization is required during the experiment, infrared sterilizer is highly recommended.

The UV lamp can only be turned on when the front window is closed and the fluorescent lamp is off.When UV lamp working avoid looking straight.

ULPA filter life: With the usage time increasing, dust and bacteria accumulate inside ULPA filter. Filter Resistance is getting bigger, when it reaches the maximum point, there will be audible and visual alarm. Please replace new ULPA filter, otherwise it will affect the safety performance of the equipment. The used filter should be processed as medical waste.

There is a negative passage surrounding the work area, which is sealed strictly in the factory. The operator is not allowed to remove or loose screws of those parts. If necessary, please contact service personal.

Front Grille is used for air intake and drain. Do not block it, otherwise it will affect airflow. Armrest is recommended to solve this problem and reducing the operator's wrist fatigue.

Long-term use of Microbiological Safety Cabinets will inevitably cause pollution (e.g. ULPA filters, corner cabinets, etc.). In order to sterilize thoroughly every 500 hours, formalin (formaldehyde) fumigation sterilizer is recommended. After sterilization, neutralize formaldehyde gas with ammonium hydrogen carbonate. Make sure no sterilization gas escapes during the whole process.

The maximum storage period is one year. If the period is more than one year, performance test should be done.

The transportation process should take appropriate protective measures according to diagram on the surface of package. Purchaser in receiving should carefully check the integrity of the box. if there is damage and crush, please refuse to accept. And timely contact with our company.

When Microbiological Safety Cabinet installed and used move again, please timely contact with our company.

The ground bearing requirements: ground bearing capacity ≥350KPa.

Gross weight: 165kg.

Allowable pressure of water socket: ≤0.8MPa. (Optional)

For experiments that produce waste liquid, please timely drain wastewater in the sump through drain valve. If the liquid waste is harmful to human's body, according to the grade of pollution should add the corresponding grade container, or connect to the corresponding grade drainpipes, and drain waste into the experimental equipment to immediately dispose.

Person can operate Microbiological Safety Cabinet only if he is qualified after training, during using Microbiological Safety Cabinet if it is a harmful experiment, operator must wear appropriate protective gloves, masks and lab coats, etc., and avoid touching mouth, eyes and face.

There is risk to move the parts moving. Relevant personnel can use this equipment must after training. while the front window must be raised to 200mm and in this case person can operate in the cabinet. In this way we can reduce harm out of front window trouble.

When the equipment is repaired or scrapped. The internal parts can't be thrown about because of biological hazards. please deal with them according to local regulations.

Serious declaration: we will take no responsibility for risks caused by improper operation and man-made damages!

2. Warranty

1) Warranty is 12 months from EX-factory date (excluding consumable accessories and Fluorescent lamp, fuse).

2) We will take no responsibility for risks caused by improper operation and man-made damages.

3) After the expiration of warranty, our company is also responsible for repairs, but the corresponding maintenance cost should be charged.

4) Lifetime of Microbiological Safety Cabinet is 8 years from production date on the label.

5) We can provide equipment drawings and necessary technical data for maintenance companies or personnel trained by our company.

Warranty declaration: One-year Warranty, Life-long Maintenance.

3. Unpacking, Installation, Debugging

Please firstly check if packing box is in good condition. If the packing box is damaged, please take photos.

3.1 Unpacking

Choose the proper unpacking method according to the actual situation.

1) Method 1: Use M8 Wrench to unpack

2) Method 2: Electric drill with hexagon dead M8

Figure 1

Figure 2

Rapid unpacking diagram . Disassemble the screws shown in the below picture , then move the

wooden pieces to right and left.

Figure 3

Unpacking method for carton

Using ordinary scissors to cut packing tape, take off the package cover, then move up the paper box body.

Figure 4

3.2 Accessories Checking

Refer to the packing list and check the accessories.

BCBS-1205 Packing List

Table 1

3.3 Installation Conditions & Working Environment

Biological safety cabinet should be placed in a protected zone of airflow, front window operation mouth cannot face Windows and doors, Away from the ventilation system and air conditioning vents, prevented from ventilation systems, air-conditioned, doors, windows and people move caused an airflow impact on the safety cabinet. The tests show that if the air flow interference than other cabinet inlet suction air velocity, indoor infection air will enter the work space of biological safety cabinet. So the correct place safety cabinet, make its in the correct position, it is very necessary. It should also pay attention to safety cabinet exhaust and indoor and ventilated air or exhaust pipe, the relationship between safety cabinet exhaust air from above the cabinet put oneself in another position, at the time of placing safety cabinet should avoid limiting exhaust it. Biological safety cabinet should be in the direction of air flow downstream, must be in the safe cabinet side to keep at least 300 mm of space, in order to check.

Working environment:

1. Only is suitable for indoor;

2. Ambient temperature: 15°C~35°C;

3. Relative Humidity:≤75%;

4. Atmospheric pressure range: 70 kPa~106 kPa;

5. Electrical parameters: Consistent with the rated voltage of the biological safety cabinet

6. Power supply need to be grounded; (Judging method: testing the live line and the zero line of the power supply with multimeter, the live line to ground voltage should be grid voltage and the zero line to ground voltage should be 0, otherwise the power supply ground is bad);

7. Installation location: The biosafety cabinet should not be located in the passage, away from the room air flow that can destroy the isolation layer generated by the working air barrier. Should not be placed in the laboratory return air outlet, affect the laboratory return air; If the laboratory has Windows, they should be closed at all times. The biosafety cabinet should not be placed in the circulation air inlet, so that air can blow through the front window operating port or blow the exhaust filter; If space permits, a 30cm space should be left behind and around the biosafety cabinet for cleaning. If not permitted, 5cm on the back should be left for cleaning the biosafety cabinet. The power socket of the biosafety cabinet can be approached to facilitate the maintenance of the biosafety cabinet, and the biosafety cabinet can be tested for electrical safety without moving it; The biosafety cabinet should be installed near the laboratory exhaust outlet, that is, downstream of the indoor air flow direction, so as to facilitate the discharge of polluted air around the biosafety cabinet as soon as possible.

Figure 5

Recommended biosafety cabinet installation location in the laboratory

Note: "A" is the best position, "B" is optional. The air outlet above or near the safety cabinet should be changed to avoid the front of the safety cabinet.

Figure 6 (Biosafety cabinet dimensions)

External Size:700*650*1920(mm)

Internal Size:600*500*540(mm)

Figure 7

Allow at least 1.0 m between the cabinet and pedestrian traffic routes, thoroughfares or walkways.

Figure 8

Allow at least 30 cm clearance on both sides of the cabinet and 5cm space on the rear of the cabinet.

Figure 9

The distance between the cabinet aperture and any doorway is at least 1.5 m.

Figure 10

The distance between the side panel and any doorway is at least 1.0 m.

Figure 11

Allow at least 3m between the aperture and the aperture of an opposing cabinet, fume cupboard, etc.

Figure 12

Any large obstruction projecting beyond the plane of the front aperture should not be within 30 cm of the sides of the cabinet.

Figure 13

You should not position the cabinet in a location where there is an obstruction that affect airflow within 2 m of the front aperture.

Figure 14

The distance between cabinet aperture and the front of a bench opposite should be at least 1.5 m aperture.

Figure 15

Avoid positioning a bench at right angles to the cabinet. Any person working at the bench is likely to disturb airflow close to the cabinet.aperture.

Figure 16

A projecting bench will help minimize traffic in front of the cabinet as long as the front of the bench is situated at least 1 m from the side of the cabinet.

3.4 Installation

1. Remove all the package modules;

2. Inspect the surface of main body whether there is scratch, deformation or foreign matters;

3. Move the whole device to the final installation location;

4. Assembly of base stand, drain valve.

5. According to the operating platform which the biological safety cabinet will be placed, to judge if the biological safety cabinet is with base stand; if the drain hole has reserved installation space, then installation of drain valve or drain baffle is needed.

Figure 17

Note: Support section at the time of transport on the back of the safety cabinet, it should be removed before installation, it is forbidden to the bottom up and place or remove dumping.

1. Refer to below picture to assemble the base stand.

Figure 18

(M10*20 socket head cap screws)

Remove the M10x20 hex cylinder head bolts from the accessory box, from the left side bracket and right side bracket of the base stand to inset screw, for connecting with the connecting plate, then tighten the screws with inner hexagon wrench, referring to above picture.

Note: The base mounting bolts are fixed to the base stand. When install the base stand, except the 4 bolts marked with red circle in above picture, please remove the other bolts and install.

2. Connect base stand and main body

Refer to the picture below to connect the base stand and main body.

Figure 19

(M10*20 hexagon socket head bolt + flat washer 10 + spring washer 10, connect the cabinet and the base, and tighten.)

Take out the hex cylinder head bolt M10*20, spring washer10, flat washer10 from the accessory box, and connect the base stand and the main body as is shown in above picture.

3. Assembly of drain valve

Figure 20

Table 2

Figure 21

Take out drain valve connector, shim, drain valve connector fastening nut, rubber gasket, drain valve, assembling from up to down as are shown in above picture.

4. Assembly of drain baffle

Figure 22

Table 3

Take out drainage board I, drainage board II, flat washer 6, spring washer 6, M6 nut, assembling from up to down as above picture illustrated.

5. Adjustment of footmaster caster

Figure 23

Clockwise rotate caster' red part to low down the base feet and the cabinet height. Low down all four casters can move the cabinet. Counterclockwise rotate casters' red part can raise the base leg and cabinet height. Raise all four casters at same time can fix the cabinet. Adjust the four casters to make the cabinet stable.

3.5 Checking after Installation

First, make sure the Voltage and frequency to be same as logo showing, and then check the follows items with power on:

Table 4

4. User Instructions

4.1 Functions

4.1.1 Product Concept

Biological safety cabinet is a kind of negative pressure filtration system for protecting operator, the laboratory environment and work materials, the front opening which air flow inward have protection function for operator, the filtered laminar flow generated by vertical ULPA can protect work materials, what's more, the polluted air flow become pure after processed by ULPA filter. When it's used in microbiology experiment environment filled with volatile or toxic chemical and radionuclide, suitable exhaust hood in function have to be linked.

4.1.2 Application Range

Biological Safety Cabinet is necessary equipment in the laboratory in the search of microbiology, biomedical, DNA recombinant, animal experiment, and biological products, especially in the occasion that operator need to adopt protective measure, such as medical and health, pharmacy, medical research. Our equipment provides a safety working environment which don't have bacterial and dust in the process of bacterial culture.

4.1.3 Operating Principle/Airflow Pattern

Figure 24

4.1.4 Protected Objects

Biological safety cabinets (BSCs) are designed to protect the operator, the laboratory environment and work materials from exposure to infectious aerosols and splashes that may be generated when manipulating materials containing infectious agents, such as primary cultures, stocks and diagnostic specimens.

4.1.5 Technical Parameters

Table 5

Notes:

(1) Electric consumption power includes the power used to load the base stand (optional).

(2) Our company has right for changing the products, if we need to change and re-design, please forgives us for not notifying you.

1. Biological safety functions

Personnel protection: with the KI way, the protection factor of the front window operating port should not be less than 1 * 105;

Sample protection: microbial colony count ≤5CFU;

Cross contamination protection: microbial colony count ≤2CFU.

2. Leak-proof Cabinet

If cabinet pressurized to 500Pa, the pressure should be no less than 450 Pa after 10 min.

3. Integrity of ULPA Filter

Scan and detect the ULPA filter, the leakage rate at any point should not be >0.01%.

4. Vibration amplitude

The net vibration amplitude between frequency 10Hz and 10KHz is no more than 5μm(rms).

5. Illumination

The average illumination is no less than 650 lux, max illumination is no less than 1000lux.

6. Mechanical performance

Structure design is reasonable, high quality materials are adopted for the cabinet.

It can resist the shape of global deformation caused by external force.

The working surface will not occur permanent deformation when weight reaches 23kg.

7. Electrical properties

The voltage increases to 1390V(AC) in 5s and keep for another 5s without breakdown.

Grounding resistance ≤0.1Ω

4.2 Product Structure

Figure 25

Table 6

Figure 26

Table 7

4.2.1 Structure Introduction

1) Driving system of front window

Driving system consists of tube motor, front window, hauling sash and position switch.

2) Air filtration system

Air Filtration System is the most important system of BSC. It consists of fan, supply filter and exhaust filter. The function of Air Filtration System is transferring filtered air to work area, ensure the down flow velocity, keep cleanness of work area and exhaust gas reach the standard request.

3) UV lamp

UV lamp is inside work area. So UV lamp can well sterilize all space of work area.

4) Fluorescent lamp

The BSC is equipped with straight tube type energy-saving fluorescent lamp. It can make sure average illumination inside work area which meets standard requirements.

5) Sampling port

The sampling port reserved on the safety cabinet for testing the aerosol concentration of the upstream of the ULPA filter is located at the lower part of the worktable in the working area.The transparent tube marked with "filter upstream" is the sampling port for aerosol concentration test on the upstream of air supply filter and exhaust filter.

Figure 27

6) Power lock

When the power cord is connected to main power, switch the key for power lock, then the equipment is powered on.

7) Waterproof socket

Waterproof Sockets are located on the right side of the work area, which can be controlled by a SOCKET button.

(1) Please make sure the total load of sockets should be ≤ 500W;

(2) Waterproof connector can only be waterproof when the lid down, the socket cannot be regards as waterproof connector when the cover is opened.

8) Fuse protector

The equipment is equipped with main power fuse, waterproof socket fuse. They are located near the power cord's outlet. Fuse label is corresponding to the relevant specifications. Please refer to 3.2.

9) LCD display

LCD Crystal Display is placed in the middle of the two pieces of parallel glass liquid crystal, there are many vertical and horizontal fine wire between two pieces of glass, control rods crystal molecules change direction through electricity or not, and will have a picture lamp refraction. Low power consumption, no electromagnetic radiation, and the service life can be up to 100000 hours.

Figure 28

Large LCD display indicates detailed key parameters; it can real-time display to reflect the equipment working condition, such as effective working state of the filter, which is more intuitive.

10) Control of front window

Front window is motorized. It could be controlled by remote control, and control panel.

11) Structure

a) Biological Safety Cabinet's both sides and back area are negative pressure air channel. Make a double isolation by air curtain and cabinet between working area and external environment And the working area surrounded by negative pressure to avoid any leakage.

b) Cabinet body is built of 1.2mm cold-rolled steel with anti-powder coating. Strong and steady.

c) Work area is fully made of 304 stainless steel which looks beautiful and with corrosion resistance performance.

d) Base stand is made of cold-rolled steel with anti-powder coating.

e) Soft touch type control panel, easy to handle and beautiful appearance.

4.3 Control Panel

Figure 29

Table 8

a) LCD screen

The working status of the equipment and operation can be seen on the LCD screen

b) Soft touch button.

Main functions could be executed by touch-buttons.

Table 9

You can also complete the time adjustment and one-key timing function of the UV lamp through the combination of buttons:

Table 10

4.4 Remote Control

It is inconvenient for the users to operate from a distance. Small &lamp remote control is flexibly to be used to control all the functions of the cabinet in a distance ≤6m, 30° range. The operator can even carry it with themselves during experiment for convenience.

This remote control adopt specific chip which is featured with good anti-jamming performance, longer control distance and high control precision.

Biological safety cabinet is equipped with special UV lamp. When turning on or turning off the cabinet, sterilization time of UV lamp should be at least 30 minutes. In order to save the waiting time of turning on or turning off the cabinet, we develop reservation time function. It realizes function of automatic turning on or turning off the cabinet after the sterilization finished. Reservation time setting range is from 0 to 99 hours and 59 minutes. This function helps operators to save time and improve efficiency.

Figure 30

Table 11

A.Timed shutdown

a. When the equipment is running, press the reservation key (SUB) to set the timed shutdown, and the LCD displays off;

b.Adjust the time by pressing the+and - buttons, press the INSTALL TIMER to switch the 10 minutes' bit, hour bit and 10 hours' bit of the time, press the CONFIRM button to save the settings and start the timed shutdown;

c.Press the SUB key twice in succession to exit the timed shutdown function.

B.Reservation timing

a.Plug in the power supply, open the power lock, and select each function button (UV, FAN, SOCKET) to start the corresponding function;

b. Modify the reservation time: when the reservation function is running, press the SUB button to set the reservation opening time, and the interface displays (on). Press the INSTALL TIMER to switch the 10 minutes' bit, hour bit and 10 hours' bit of the time, and press the + and - buttons to adjust the time; Press the CONFIRM button again, and the interface displays (off). Set the running time of the reservation function. The modification steps are the same as above; Finally, press the CONFIRM button to start the reservation timing function;

c.To start the reservation function immediately, set the start time (on) to 00:00; If it is not necessary to start the reservation function immediately, after completing the corresponding reservation settings, press the corresponding buttons (UV, FAN, SOCKET) to exit, and then press the reservation function button to start the reservation function;

d.The glass door must be closed before starting the UV reservation timing function; The glass door must be at a safe height before starting the fan reservation timing function;

e.nterface on: indicates the function will be started after a period of time; Interface off: indicates the function will be closed after running a period of time.

C.Other buttons have the same function as the touch buttons on the operation panel.

4.5 Instructions for Operation

4.5.1 Normal Operation Notice

1) Make sure input voltage is correct and stable.,The rated load of main power socket should be higher than cabinet consumption. Plug must be well grounded.

2) In order to avoid air turbulence, the operator should slightly move his arms during experiment. Hands should stay inside the working area at least 1 minute before operating. In order to decrease the times of arms moving into and out of the working area, prepare all the necessary items inside the cabinet before starting experiment;

3) Moving principles of different samples inside cabinet: When two or more samples need to be moved, be sure that low-polluting samples move to high-polluting samples. Movement of items should also follow the principles of slow-moving.

4) Samples placed in parallel: Samples should be placed in the cabinet parallel to avoid cross-contamination between samples and blocking back air grille.

5) In order to avoid samples being sucked into the negative passage or the Fan, do not place soft and slight samples (for example: soft tissue) on the surface during experiment;

6) The weight of items placed in the cabinet should be no more than 23Kg/25x25cm2;

7) Avoid vibration: avoid using vibration equipment (eg centrifuges, vortex oscillator, etc.) inside the cabinet. Vibration would cause lower cleanliness of operating area and affect operator protection.

8) No flame: No flame is allowed inside the cabinet. Using of fire will lead to airflow disorder, and filter damage. If sterilization is required during the experiment, infrared sterilizer is highly recommended.

9) ULPA filter life: With the usage time increasing, dust and bacteria accumulate inside ULPA filter. Filter Resistance is getting bigger, when it reaches the maximum point, there will be audible and visual alarm. Please contact our company service department in time, replace new ULPA filter, otherwise it will affect the safety performance of the equipment. The used filter should be processed as medical waste.

10) There is a negative passage surrounding the work area, which is sealed strictly in the factory. The operator is not allowed to remove or loose screws of those parts. If necessary, please contact service personal.

11) Front Grille is used for air intake and drain. Do not block it, otherwise it will affect airflow. Armrest is recommended to solve this problem and reducing the operator's wrist fatigue.

12) Long-term use of biological safety cabinets will inevitably cause pollution (e.g. ULPA filters, corner cabinets, etc.). In order to sterilize thoroughly every 500 hours, formalin (formaldehyde) fumigation sterilizer is recommended. After sterilization, neutralize formaldehyde gas with ammonium hydrogen carbonate. Make sure no sterilization gas escapes during the whole process.

The maximum storage period is one year. If the period is more than one year, performance test should be done.

Serious declaration: we will take no responsibility for risks caused by improper operation and man-made damages!

4.5.2 Operation Process

1) Connect the same power reply, as required of equipment

2) Open the power lock, LCD display lamps up and alarm rings at the same time, then the machine enters to standby status. Waiting for the operator to input button to use it;

3) Press POWER button, then the following functions are available: Fluorescent lamp. UV lamp,

Fan, Mute, Sockets, Front window up and down, Reservation timing;

When front window is closed and fluorescent lamp is off, then press the UV button to select the sterilization function.

4) Before doing experiment, please sterilize the cabinet for more than 30 minutes by UV lamp;

(1) For safety of eyes and skin, people should leave room during the UV sterilization.

(2) UV lamp intensity should be tested regularly. If there is no test conditions, it should be replace when the UV timer on the display indicate the working time reaches to 1000 hours.

5) Please move the front window at 200mm height from the work table, turn on the fan, make sure the experiment should be started after fan working for at least half an hour;

For operating safety, please put testing materials inside the cabinet in advance, and keep the front window at 200mm height from the work table during operation.

6) After finishing the experiment, please move the front window down to the bottom, and make sure to sterilize the cabinet by UV lamp for 30 minutes before turning off the cabinet.

4.6. Daily Maintenance

Because the operating time will directly affect the judgment of maintenance needs, we recommend the user keep a detailed record of operating time for reference.